h460 lung large cell carcinoma cell line  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: Illustration of the cytotoxicity activity of Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate in (A) A549 and (B) NCI-H460 cell lines.

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: SIRT6 expression in A549 and NCI-H460 NSCLC cell lines: (A) Represents the qRT-PCR analysis and (B) Represents the Western blot analysis. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01, *** p < 0.001 and **** p < 0.0001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Gene Expression, Control

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: The effect of imidazole derivative Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate on antioxidant regulators (A) GSH Activity, (B) GPx Activity, (C) Catalase Activity, and (D) % Radical Scavenging Activity in A549 and NCI-H460 cell lines. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01, and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Activity Assay

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: The protein expression of cytochrome-c (cyt-c) in NSCLC cell lines A549 and NCI-H460. β-actin is used as an internal control for normalization. (Data represent mean values ±SD. ** p < 0.01, and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Control

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: Gene and protein expression of caspase 9: (A) Caspase 9 gene expression, (B) pro-caspase 9, and (C) active-caspase 9 protein expression in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Gene Expression, Control

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: The expression of Caspase 3: (A) Caspase 3 gene expression, (B) protein expression of pro-caspase 3, and (C) active-caspase 3 in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD.** p < 0.01, *** p < 0.001 and **** p < 0.0001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Gene Expression, Control

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: Morphological assessment of NSCLC cell lines A549 and NCI-H460 cells stained by (A) AO/EtBr, (B) Propidium iodide, (C) DCFH-DA, (D) Rhodamine-123, and (E) Hoechst 33258 were captured using a fluorescent microscope with ×20 magnification (Scale Bar - 125 μm). (F) AO/EtBr, (G) Propidium iodide, (H) DCFH-DA, (I) Rhodamine-123, and (J) Hoechst 33258 represent the analysis of morphological staining by image (J) (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Staining, Microscopy

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: Gene and protein expression of Keap1 in A549 and NCI-H460 NSCLC cell lines: (A) Keap1 gene expression and (B) Western blot analysis of Keap1. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01 and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Gene Expression, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

doi: 10.3389/fphar.2023.1335305

Figure Lengend Snippet: Gene and protein expression of Nrf2 in A549 and NCI-H460 NSCLC cell lines: (A) Nrf2 gene expression and (B) Western blot analysis of Nrf2. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Gene Expression, Western Blot, Control